In vitro propagation, lactucin quantification, and antibacterial activity of Indian lettuce (Lactuca indica L.)

Abstract Lactuca indica L. (Asteraceae), a wild lettuce, is used as vegetable and in traditional medicine. This study aims to establish vitro propagation protocol evaluate lactucin antibacterial property from natural plant tissues. Leaf blades petioles were cultured on Murashige Skoog (MS) medium supplemented with various concentrations of 6-benzylaminopurine (BAP) 1.2 mg L –1 indoleacetic acid (IAA). lower BAP concentration (0.5 ) optimal for direct shoot induction, while the leaf blade higher (4 performed best callus induction. When was subcultured, 98.7% samples regenerated plants MS 1.5 0.5 IAA. containing 1 IAA rooting. A high-performance liquid chromatography analysis revealed amount (sesquiterpene lactones) root than leaf, whereas naturally grown plants, amounts obtained juvenile followed by flowering lowest concentration. All tissue extracts showed activity against Pseudomonas fuscovaginae (a rice pathogen) Escherichia coli , which directly proportional produced. regeneration phytochemical investigation will facilitate further exploitation this useful plant.